How To Properly Measure Peptides For Your Research?

Posted on October 9, 2016 By

peptidesSynthetic Peptides are popular ingredients that are currently being researched the world over. Several young enthusiastic scientists are also conducting their own research with these chemicals. So if you are one of these young enthusiasts, who is looking for pure peptides for your research, then you need not worry. There are several online sellers who supply you with your chemicals. Check out americansciencelabs.com for all your research peptide needs. If you are wondering what are peptides, then is the right place to begin your journey, http://www.academia.edu/5605402/Amino_acid_peptides_and_proteins.

Just as the purity of your peptides can make or break the success of your experiment, the correct dosage also plays a vital role in the final outcome. Measuring the right dosage is difficult. This is because of minute measurements that are usually done in very small quantities like micrograms. The best way to go about the measurements is by planning the reconstruction numbers for the peptides before you begin the experiment. Peptides are measured in a quantity called as aliquots that typically measure in 1mg, 2mg, 5mg and 10mg.

You can also use the peptide calculator tools to help you arrive at the exact measurements for your research purposes. You must make sure that you check your measurements more than once before you begin.

The following synthetic peptides like Melanotan, GRHP 6 and CJC are fairly easy to reconstitute. Peptides are usually shipped in lyophilized form at room temperatures without the need for cold storage. They are then reconstituted easily using bact water. Make sure that you use distilled water for all your reconstitution purposes.

If you are a visual learner, you can familiarize yourself with the process of reconstitution by watching videos on YouTube. There are several channels that provide you with useful information that helps you to learn the right way to mix peptides and how to use peptide calculators to measure exactly for your research.

How to dissolve Peptides?
The solubility of your synthetic peptides is dependent on its polarity. Acidic peptides can be dissolved in basic solutions whereas basic peptides are dissolved in acidic buffers. Sonication is a process used to increase the solubility of a peptide. You can determine the success of your peptide solution on the basis of a simple naked eye test. If the solution appears cloudy or white, then it means that the solution is not perfect. A rightly mixed solution is clear and highly transparent.

Hydrophobic peptides are highly reactive. It crystallizes as soon as it comes into contact with the air. So make sure that you don’t dissolve this category of peptides in distilled water. Instead make use of an organic solvent like acetic acid, that doesn’t react with hydrophobic or neutral peptides.

To reduce the degradation of your peptide sample make sure that you store it at temperatures lesser that -20 degree Celsius. Also do not subject your chemicals to repeated thaw and freeze cycles. Instead divide the sample into individual aliquots to make sure they have a long shelf life.

We hope this article enlightened you in the right way to measure peptides for your research.

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